DNA Sequencing: From Experimental Methods to Bioinformatics

摘要: What is DNA Sequencing? Chemical degradation (Maxam and Gilbert) method. Chain termination (Sanger dideoxy) Instrumentation reagents. Template preparation. Gel electrophoresis. Non-radioactive methods. Troubleshooting. Confirmatory sequencing. Sequencing PCR products. Strategies for new sequence determination. Introduction to Bioinformatics the Internet. Sequence databases. alignment database searches. projects Contig analysis. Protein function prediction. structure Appendices.