Homologous recombination in the oocyte
作者： Ralf Kühn , Melanie Meyer , Wolfgang Wurst
DOI:
关键词: Nucleic acid sequence 、 Oocyte 、 Biochemistry 、 Double strand 、 Homologous chromosome 、 Sequence (medicine) 、 Homologous recombination 、 Biology 、 Genome 、 Molecular biology 、 Zinc finger nuclease
摘要: The present invention relates to a method of modifying target sequence in the genome mammalian or avian oocyte by homologous recombination with donor nucleic acid sequence, comprising steps (a) introducing into zinc finger nuclease molecule encoding expressible form, wherein specifically binds within and introduces double strand break sequence; (b) oocyte, comprises regions sequence. further producing non-human mammal an carrying modified its genome.
lens.org参考文章(48)
J. Y. Nothias, M. Miranda, M. L. DePamphilis, Uncoupling of transcription and translation during zygotic gene activation in the mouse. The EMBO Journal. ,vol. 15, pp. 5715- 5725 ,(1996) , 10.1002/J.1460-2075.1996.TB00955.X
Masamichi Kamihira, Ken-ichi Nishijima, Shinji Iijima, Transgenic Birds for the Production of Recombinant Proteins Recent Progress of Biochemical and Biomedical Engineering in Japan II. ,vol. 91, pp. 171- 189 ,(2004) , 10.1007/B94209
A mouse for all reasons. Cell. ,vol. 128, pp. 9- 13 ,(2007) , 10.1016/J.CELL.2006.12.018
Fyodor Urnov, Aron M. Geurts, Xiaoxia Cui, Genome editing in rats using zinc-finger nucleases ,(2009)
Luke Alphey, DNA Sequencing: From Experimental Methods to Bioinformatics ,(1997)
J. Craig Venter, Chris Fields, Mark D. Adams, Automated DNA sequencing and analysis. Academic Press. ,(1994)
Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy, Richard Behringer, Manipulating the Mouse Embryo: A Laboratory Manual ,(2002)
Raul M Torres, Ralf Kühn, Laboratory Protocols for Conditional Gene Targeting ,(1997)
Alexandra L. Joyner, Gene Targeting: A Practical Approach ,(2021)
Philippe Soriano, Generalized lacZ expression with the ROSA26 Cre reporter strain Nature Genetics. ,vol. 21, pp. 70- 71 ,(1999) , 10.1038/5007