Uncoupling of transcription and translation during zygotic gene activation in the mouse.

摘要: Zygotic gene expression in mice is delayed by a time-dependent mechanism until the two-cell stage development. To investigate basis of this 'zygotic clock', firefly luciferase was injected into mouse embryos, and quantitative assays were used to monitor transcription translation individual embryos from single mothers. These studies confirmed, at mRNA level, previous conclusions about relative capacities paternal maternal pronuclei transcribe genes, requirements for promoters enhancers during zygotic activation. Furthermore, these revealed that fertilized eggs can delay genes uncoupling transcription. An RNA polymerase II-dependent could be translated began (a up 15 h after injection). The time course nascent accumulation biphasic, with second phase occurring expression. If had begun, then tightly linked repressed, not produced. Therefore, onset Similar biphasic courses also observed I- III-dependent other results reveal clock regulates both genes.