Translation and stability of ovalbumin messenger RNA injected into growing oocytes and fertilized ova of mice.
作者： Brinster Rl , Ebert Km , Paynton Bv , McKnight Gs
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摘要: Growing mouse oocytes and fertilized ova were injected with chicken ovalbumin messenger RNA (mRNA ov ) conalbumin mRNA con cultured in vitro . Estimation of stability by hybridization cDNA to extracted from indicated a half-life 147 366 h the oocyte 5 3 ovum respectively. Stability was similar unfertilized ovum. Oocytes also labelled [ H]leucine synthesis measured immunoprecipitation. The amount synthesized during initial 7 less than period 18–25 or 66–73 postinjection. greatest percentage total protein occurred between h. secreted 12% each periods (0–7, h) indicating stable mechanism for secretion throughout culture period. These studies demonstrate: (1) dramatic difference growing ovum, (2) gradual increase translation culture.
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