Secretion of proteins by the fertilized mouse ovum

摘要: Abstract Fertilized one-cell mouse ova were injected with messenger RNA (mRNA) for chicken ovalbumin or rat albumin. The labeled [ 3 H]amino acids, and endogenous proteins as well in the culture medium separated by two-dimensional electrophoresis. Following injection of either message, able to synthesize secrete appropriate protein. However, when message globin was injected, appeared only among ovum but not secreted into medium. location proteins, albumin ovalbumin, on gels suggests that process before secretion removing amino-terminal signal sequence from glycosylating ovalbumin. Rat may be exported more rapidly than In addition, at least one protein stage. studies establish ability newly fertilized messages.