Laboratory Protocols for Conditional Gene Targeting

摘要: Section 1: The Cre-loxP recombination system and gene targeting in ES cells 1. Gene strategies 2. 3. vectors - consideration use of loxP 4. Nonselectablae modifications removal selection marker genes 5. Large deletions 6. replacement 7. Targeted integrations 9. Conditional modification 10. LoxP-containing transgenes 11. 2: Working with 12. cell transfection overview 13. Embryonic feeder 14. handling 15. culture 16. clone picking 17. Identification homologous recombinants 18. Freezing clones 19. Thawing 20. Genomic DNA preparation 21. Cre-mediated neomycin deletion 22. Generation mice 23. Mouse breeding for injection or aggregation 24. Injection needles holders 25. Blastocyst preparation/injection 26. Embryo 27. transfer Appendices References Selected references further reading