Diagnosis of acute megakaryoblastic leukemia by flow cytometry and immunoalkaline phosphatase techniques. Utilization of new monoclonal antibodies.

摘要: This report demonstrates a case of acute megakaryoblastic leukemia evolving in patient with idiopathic myelofibrosis myeloid metaplasia. A presumptive diagnosis was made by cytochemical stains, alpha-naphthyl acetate esterase, and butyrate esterase. The established electron microscopic platelet peroxidase studies the peripheral blood blast cells supported flow cytometry immunoalkaline phosphatase studies. Specific monoclonal antibodies directed against glycoproteins Ib (6D1) IIb/IIIa (10E5), which have not been described frequently analyzing leukemia, were used for direct technique. Cytogenetic demonstrated deletion long arm chromosome 6 breakpoints at bands q15 q23, ring (p25, q27). requires accurate examination, employing specific platelets megakaryoblasts. exact origin circulating megakaryoblasts these cases is known needs additional investigation.