Electromagnetic fields with 217 Hz and 0.2 mT as hazardous factors for tubulin structure and assembly (in vitro study)
作者： Ali Afrasiabi , Gholam Hossein Riazi , Ali Dadras , Elaheh Tavili , Behafarid Ghalandari
DOI: 10.1007/S13738-013-0398-Y
关键词:
摘要: Nowadays exposure to extremely low frequency electromagnetic fields (ELF-EMFs) is an unavoidable fact in human societies. In spite of destructive effects ELF-EMF such as impression on short-term memory and cognitive performances, neuroprotective neurogeneration have been reported. On the other hand, microtubule (MT) proteins, dynamic cytoskeleton proteins that are mostly located axons, able produce local EMFs possess key role formation consciousness. Therefore, MTs strong potential be affected by exogenous ELF-EMFs. present study, decline MT polymerization was shown transmission electron microscopy images turbidimetry method after ELF-EMFs with 50, 100, 217 Hz frequencies at 0.2 mT intensity. Circular dichroism 8-anilino-1-naphthalenesulfonate (ANS) fluorometry were used demonstrate secondary third structural changes. Decrement through disruption tubulin native structure, increasing β-sheets random coils observed all conditions. specially could lead functions neural cells.
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