Antimicrobial Activity of Soil Clostridium Enriched Conditioned Media Against Bacillus mycoides, Bacillus cereus, and Pseudomonas aeruginosa.

摘要: The rise of antimicrobial resistant bacteria has fast-tracked the exploration for novel compounds. Reports on producing soil anaerobes such as Clostridium spp. are very limited. In present study, activity enriched conditioned/spent media (CMs) against Bacillus mycoides, cereus and Pseudomonas aeruginosa was assessed by turbidimetric growth inhibition assay. Our results highlighted potential conditioned pathogenic spoilage bacteria. Farm 4 medium (F4SCM) demonstrated a greater all three tested microorganisms in comparison to other media. Non-targeted metabolite profiling revealed distinctive polar intermediate-polar metabolites F4SCM, consistent with its strong property. Moreover, 539 significantly abundant including some unique features were detected F4SCM suggesting substantial specialized chemical diversity. This study putatively identified seven high F4SCM; 3-hydroxyphenylacetic acid, γ-aminobutyric creatine, tryptamine, 2-hydroxyisocaproic acid. Tryptamine acid previously reported have properties. shows that promising group provides future prospects clostridial discovery within their metabolic