[58] Determination of the tryptophan content of proteins with N-bromosuccinimide

作者: T.F. Spande , B. Witkop

DOI: 10.1016/S0076-6879(67)11060-4

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摘要: Publisher Summary A rapid and convenient spectrophotometric method for the estimation of tryptophan in proteins, utilizing the reagent N-bromosuccinimide (NBS) is discussed. This …

参考文章(15)
Nancy Konigsberg, George Stevenson, J. Murray Luck, Further studies on the bromodecarboxylation of amino acids with N-bromosuccinimide. Journal of Biological Chemistry. ,vol. 235, pp. 1341- 1345 ,(1960) , 10.1016/S0021-9258(18)69409-8
G.H. Beaven, E.R. Holiday, Ultraviolet absorption spectra of proteins and amino acids. Advances in Protein Chemistry. ,vol. 7, pp. 319- 386 ,(1952) , 10.1016/S0065-3233(08)60022-4
YOSHIMI OKADA, KAORU ONOUE, SHOICHI NAKASHIMA, YUICHI YAMAMURA, STUDIES ON ENZYME-ANTIENZYME SYSTEMS. II. N-BROMOSUCCINIMIDE MODIFIED BACTERIAL ALPHA-AMYLASE. Journal of Biochemistry. ,vol. 54, pp. 477- 483 ,(1963) , 10.1093/OXFORDJOURNALS.JBCHEM.A127819
NM GREEN, AVIDIN. 3. THE NATURE OF THE BIOTIN-BINDING SITE. Biochemical Journal. ,vol. 89, pp. 599- 609 ,(1963) , 10.1042/BJ0890599
W. L. Bencze, K. Schmid, Determination of Tyrosine and Tryptophan in Proteins Analytical Chemistry. ,vol. 29, pp. 1193- 1196 ,(1957) , 10.1021/AC60128A025
G.J.S. Rao, H.R. Cama, Action of N-bromosuccinimide on human hemoglobin and its possible bearing on heme-globin linkage. Biochimica et Biophysica Acta. ,vol. 71, pp. 139- 149 ,(1963) , 10.1016/0006-3002(63)90992-2
J. R. Spies, D. C. Chambers, Chemical Determination of Tryptophan in Proteins Analytical Chemistry. ,vol. 21, pp. 1249- 1266 ,(1949) , 10.1021/AC60034A033
F. A. Quiocho, M. O'Dell, F. Friedberg, N-bromosuccinimide action on creatine phosphokinase Cellular and Molecular Life Sciences. ,vol. 17, pp. 217- 218 ,(1961) , 10.1007/BF02160624
T. Viswanatha, W.B. Lawson, The action of N-bromosuc-cinimide on chymotrypsin. Archives of Biochemistry and Biophysics. ,vol. 93, pp. 128- 134 ,(1961) , 10.1016/0003-9861(61)90324-1
T. W. Goodwin, R. A. Morton, The spectrophotometric determination of tyrosine and tryptophan in proteins. Biochemical Journal. ,vol. 40, pp. 628- 632 ,(1946) , 10.1042/BJ0400628