Immunohistochemical Study of Calpain-Mediated α-Crystallin Proteolysis in the UPL Rat Hereditary Cataract
作者： M Tomohiro
DOI: 10.1016/S0021-5155(97)00029-4
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摘要: Abstract The UPL (Upjohn Pharmaceutical Limited) rat is a dominant hereditary cataract model that develops early-onset cataracts (E-type) in rats homozygous for the trait, and late-onset (L-type) heterozygous rats. Using antibodies specific to calpain-proteolyzed forms of α-crystallin, we determined their immunohistochemical localization L- E-rat lenses. Immunoreactivity indicating proteolyzed was detected found restricted degenerated lens fibers mature stage L-rat cataract. Lenses from E-rats, which have abnormally elongated during fetal period, had α-crystallin at 1 week age. results this present study indicate calpain-mediated proteolysis occurred lenses formation calpain may be an important factor development complete opacification.
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