Mechanism for the protective effects of silymarin against carbon tetrachloride-induced lipid peroxidation and hepatotoxicity in mice. Evidence that silymarin acts both as an inhibitor of metabolic activation and as a chain-breaking antioxidant.

摘要: Abstract Administration of silymarin (800 mg/kg i.p.) 30 min before carbon tetrachloride (18 μL/kg did not modify total hepatic levels CCl 4 and metabolites in mice, but decreased by 40% the vivo covalent binding to lipids at 2 hr. This pretreatment 60% exhalation ethane during first hour after , 50% incidence liver cell necrosis. In vitro μg/mL) 50 70% various monooxygenase activities, 20% microsomal proteins. Silymarin lipid peroxidation mediated metabolites, 90% NADPH alone. Silibinin, one three isomers composing silymarin, also tetrachloride-induced peroxidation; this effect, however, was less than that more transient . Pretreatment with silibinin 30min (18μL/kg improve SGPT activity or histology 24 We conclude prevents hepatotoxicity firstly, decreasing metabolic activation and, secondly, acting as a chain-breaking antioxidant.