Characterization of genetic interactions with RFA1: the role of RPA in DNA replication and telomere maintenance.

作者: Julianne Smith , Hui Zou , Rodney Rothstein

DOI: 10.1016/S0300-9084(00)00183-8

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摘要: Abstract Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding whose role in replication, recombination and repair has been mainly elucidated through vitro biochemical studies utilizing the mammalian complex. However, identification of homologs all three subunits Saccharomyces cerevisiae offers opportunity examining vivo RPA. In our laboratory, we have previously isolated missense allele RFA1 gene, encoding p70 subunit RPA complex . Strains containing this mutant allele, rfa1-D228Y , display increased levels direct-repeat recombination, decreased heteroallelic UV sensitivity S-phase delay. study, characterized further by screening other replication mutants for synthetic lethal phenotype combination with allele. Among examined, only one displayed phenotype, pol12-100 conditional B pol α-primase. addition, delayed senescence was observed strains null mutation HDF1 S. homolog 70 kDa Ku. Interestingly, synergistic reduction telomere length double suggests that shortening telomeres may be cause viability these strains. Furthermore, result represents first evidence maintenance.

参考文章(64)
A. Boulet, M. Simon, G. Faye, G. A. Bauer, P. M. Burgers, Structure and function of the Saccharomyces cerevisiae CDC2 gene encoding the large subunit of DNA polymerase III. The EMBO Journal. ,vol. 8, pp. 1849- 1854 ,(1989) , 10.1002/J.1460-2075.1989.TB03580.X
Fred Sherman, Getting started with yeast. Methods in Enzymology. ,vol. 350, pp. 3- 41 ,(2002) , 10.1016/S0076-6879(02)50954-X
H. Feldmann, E.L. Winnacker, A putative homologue of the human autoantigen Ku from Saccharomyces cerevisiae Journal of Biological Chemistry. ,vol. 268, pp. 12895- 12900 ,(1993) , 10.1016/S0021-9258(18)31470-4
Fred Sherman, Gerald R. Fink, James B. Hicks, Methods in yeast genetics Cold Spring Harbor Laboratory. ,(1979)
M. P. Fairman, B. Stillman, Cellular factors required for multiple stages of SV40 DNA replication in vitro. The EMBO Journal. ,vol. 7, pp. 1211- 1218 ,(1988) , 10.1002/J.1460-2075.1988.TB02933.X
J Hurwitz, M K Kenny, U Schlegel, H Furneaux, The role of human single-stranded DNA binding protein and its individual subunits in simian virus 40 DNA replication. Journal of Biological Chemistry. ,vol. 265, pp. 7693- 7700 ,(1990) , 10.1016/S0021-9258(19)39170-7
S.H. Lee, Z.Q. Pan, A.D. Kwong, P.M. Burgers, J. Hurwitz, Synthesis of DNA by DNA polymerase epsilon in vitro. Journal of Biological Chemistry. ,vol. 266, pp. 22707- 22717 ,(1991) , 10.1016/S0021-9258(18)54626-3
I. Dornreiter, L.F. Erdile, I.U. Gilbert, D. von Winkler, T.J. Kelly, E. Fanning, Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen. The EMBO Journal. ,vol. 11, pp. 769- 776 ,(1992) , 10.1002/J.1460-2075.1992.TB05110.X