作者: Julianne Smith , Hui Zou , Rodney Rothstein
DOI: 10.1016/S0300-9084(00)00183-8
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摘要: Abstract Replication protein A (RPA) is a heterotrimeric single-stranded DNA binding whose role in replication, recombination and repair has been mainly elucidated through vitro biochemical studies utilizing the mammalian complex. However, identification of homologs all three subunits Saccharomyces cerevisiae offers opportunity examining vivo RPA. In our laboratory, we have previously isolated missense allele RFA1 gene, encoding p70 subunit RPA complex . Strains containing this mutant allele, rfa1-D228Y , display increased levels direct-repeat recombination, decreased heteroallelic UV sensitivity S-phase delay. study, characterized further by screening other replication mutants for synthetic lethal phenotype combination with allele. Among examined, only one displayed phenotype, pol12-100 conditional B pol α-primase. addition, delayed senescence was observed strains null mutation HDF1 S. homolog 70 kDa Ku. Interestingly, synergistic reduction telomere length double suggests that shortening telomeres may be cause viability these strains. Furthermore, result represents first evidence maintenance.