Bone Marrow Cell Differentiation Induced by Mechanically Damaged Osteocytes in 3D Gel‐Embedded Culture

摘要: Osteocytes are suggested to have a crucial role in the initial resorptive phase of bone turnover after microdamage. To study osteocytes targeted remodeling, we developed an vitro model, which can be locally damaged and their interactions with marrow cells studied. Our results show that activate osteoclast precursors by soluble factors thus control remodeling. Introduction: Microdamage contributes fractures acts as stimulus for remodeling. Besides some remodeling may also random serve metabolic purposes. been considered provide activation osteoclastic resorption adjacent site. This was aimed develop relevant model induce stage. Materials Methods: We new device, osteocyte-like cell line MLO-Y4 were 3D cultured, subjected local scratching, assayed viability. NIH3T3-3 used control. Bone cultured on top mechanically cells, formation TRACP+ assayed. Additionally, conditioned medium from scratched cultures added cultures, TRACP activity lysates quantified. The macrophage-colony stimulating factor (M-CSF) RANKL secretion ELISA. Results: Scratching induced death cells. When over gel-embedded application mechanical scratching differentiation gel surface. could observed very restricted region along path. secreted M-CSF RANKL, showed potential culture. Conclusions: These findings indicate formation. directly shows association between initiation stage