Comparative study of in vitro and in vivo modulation of lactogenic and somatotropic receptors by native human growth hormone and its modified analog prepared by recombinant deoxyribonucleic acid technology
作者： AVI ASHKENAZI , TIKVA VOGEL , ITAMAR BARASH , DAN HADARI , AVIGDOR LEVANON
关键词:
摘要: A modified analog of human GH (hGH), prepared by recombinant DNA technology, that lacks 13 amino acids at the terminus (Met14 hGH), was able to compete with [125I]hGH for binding lactogenic receptors in Nb2-llC rat lymphoma cells, somatotropic IM-9 lymphocytes, and both microsomal fraction virgin female liver. Exposure intact Nb2 or cells Met14hGH did not reduce number surface intracellular receptors, as compared control without hormone. parallel exposure 500-fold lower concentrations hGH resulted 77–93% reduction receptors. In contrast [125I]hGH, [125I]Met uhGH taken up cells. Infusion anesthetized rats 3 h down-regulated similar infusion 200-fold higher amounts MetI4hGH total recep...
oup.com
sci-hub.se 参考文章(18)
M A Lesniak, J Roth, Regulation of receptor concentration by homologous hormone. Effect of human growth hormone on its receptor in IM-9 lymphocytes. Journal of Biological Chemistry. ,vol. 251, pp. 3720- 3729 ,(1976) , 10.1016/S0021-9258(17)33403-8
Alexander Levitzki, Receptors: A Quantitative Approach ,(1984)
I. Barash, Z. Madar, A. Gertler, Down-regulation of prolactin receptors in the liver, mammary gland and kidney of female virgin rat, infused with ovine prolactin or human growth hormone Biochemical and Biophysical Research Communications. ,vol. 116, pp. 644- 650 ,(1983) , 10.1016/0006-291X(83)90573-9
George Scatchard, The Attractions of Proteins for Small Molecules and Ions Annals of the New York Academy of Sciences. ,vol. 51, pp. 660- 672 ,(1949) , 10.1111/J.1749-6632.1949.TB27297.X
MAXINE A. LESNIAK, JESSE ROTH, PHILLIP GORDEN, JAMES R. GAVIN, Human Growth Hormone Radioreceptor Assay using Cultured Human Lymphocytes Nature. ,vol. 241, pp. 20- 22 ,(1973) , 10.1038/NEWBIO241020A0
ARIEH GERTLER, AVI SHAMAY, NAVA COHEN, AVI ASHKENAZI, HENRY G. FRIESEN, AVIGDOR LEVANON, MARIAN GORECKI, HAIM AVIV, DAN HADARY, TIKVA VOGEL, Inhibition of Lactogenic Activities of Ovine Prolactin and Human Growth Hormone (hGH) by a Novel Form of a Modified Recombinant hGH Endocrinology. ,vol. 118, pp. 720- 726 ,(1986) , 10.1210/ENDO-118-2-720
J. Schlessinger, Y. Shechter, M. C. Willingham, I. Pastan, Direct visualization of binding, aggregation, and internalization of insulin and epidermal growth factor on living fibroblastic cells. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 75, pp. 2659- 2663 ,(1978) , 10.1073/PNAS.75.6.2659
CHARLES S. NICOLL, GREGG L. MAYER, SHARON M. RUSSELL, Structural features of prolactins and growth hormones that can be related to their biological properties. Endocrine Reviews. ,vol. 7, pp. 169- 203 ,(1986) , 10.1210/EDRV-7-2-169
L. Beguinot, R. M. Lyall, M. C. Willingham, I. Pastan, Down-regulation of the epidermal growth factor receptor in KB cells is due to receptor internalization and subsequent degradation in lysosomes Proceedings of the National Academy of Sciences of the United States of America. ,vol. 81, pp. 2384- 2388 ,(1984) , 10.1073/PNAS.81.8.2384
A. Gertler, A. Ashkenazi, Z. Madar, Binding sites of human growth hormone and ovine and bovine prolactins in the mammary gland and the liver of lactating dairy cow Molecular and Cellular Endocrinology. ,vol. 34, pp. 51- 57 ,(1984) , 10.1016/0303-7207(84)90158-8