Enzyme immunoassay using peroxidase as a label and a dip-strip test for monitoring residual bacitracin in chicken plasma

摘要: A sensitive enzyme linked immunosorbent assay (ELISA) using horseradish peroxidase as a label and simple dip-strip test this ELISA which enable the monitoring of bacitracin (BC) in chicken plasma are described. The anti-BC antiserum was raised against antigenic BC-bovine gamma globulin conjugate rabbit possessed an excellent specificity for BC, exhibiting no cross-reactivity with other antibiotics used animal feed additives veterinary drugs. detection limit BC by estimated to be 0.1 μg l−1 (equivalent 1 ng per ml plasma). recoveries from ranged 97% 103%. IgG dotted nitrocellulose membrane prepared rapid developed. Using test, 50 could distinguished that control (blank) plasma.