Proteomic analysis of Chromobacterium violaceum and its adaptability to stress

摘要: Chromobacterium violaceum (C. violaceum) occurs abundantly in a variety of ecosystems, including ecosystems that place the bacterium under stress. This study assessed adaptability C. by submitting it to nutritional and pH stresses then analyzing protein expression using bi-dimensional electrophoresis (2-DE) Maldi mass spectrometry. grew best neutral, nutrient-rich medium (reference conditions); however, total recovered from stressed bacteria cultures was always higher than our reference culture. The diversity proteins expressed (repressed number identifiable 2-DE spots) seen be highest cultures, suggesting stress reduces overall range violaceum. Database comparisons allowed 43 55 spots subjected spectrometry characterized as containing single protein. Stress-related changes were noted for related previously bacterial proteins: DnaK, GroEL-2, Rhs, EF-Tu, EF-P; MCP, homogentisate 1,2-dioxygenase, Arginine deiminase ATP synthase β-subunit well ribosomal subunits L1, L3, L5 L6. ability adapt its cellular mechanics sub-optimal growth production conditions illustrated regulation subunits. With exception subunit which plays role folding maybe therefore more useful stressful conditions, all other have reduced cultures. Curiously, violeaceum also observed lose their violet color stress, suggests violacein pigment biosynthetic pathway is affected Analysis proteomic signatures indicates nutrient-starvation can cause receptors, transporters, involved with pathways, molecule recycling, energy production. Our findings complement recent publication genome sequence could help future commercial exploitation violeaceum.