Gene expression in Chromobacterium violaceum

摘要: The repertoire of 4,431 open reading frames (ORFs), eight rRNA operons and 98 tRNA genes Chromobacterium violaceum must be expressed in a regulated manner for successful adaptation to wide variety environmental conditions. To accomplish this feat, the organism relies on protein machineries involved transcription, RNA processing translation. Analysis C. genome showed that transcription initiation, elongation termination are performed by five well-known polymerase subunits, categories sigma 70 factors, one 54 factor, as well six auxiliary factors. is endonucleases exonucleases, such ribonuclease H, E, P, III, addition poly(A) specific methyltransferases pseudouridine synthases. ORFs all ribosomal proteins, except S22, were found. Only 19 aminoacyl-tRNA synthetases found, three synthetase-related proteins. Asparaginyl-tRNA (Asn) probably obtained enzymatic modification mischarged aminoacyl-tRNA. translation factors IF-1, IF-2, IF-3, EF-Ts, EF-Tu, EF-G, RF-1, RF-2 RF-3 present genome, although absence selB suggests does not synthesize selenoproteins. components trans-translation, tmRNA associated genome. Finally, large number related regulation gene expression also which was expected, considering apparent adaptability bacterium.