Primate-specific RFPL1 gene controls cell-cycle progression through cyclin B1/Cdc2 degradation.

摘要: Ret finger protein-like 1 (RFPL1) is a primate-specific target gene of Pax6, key transcription factor for pancreas, eye and neocortex development. However, its cellular activity remains elusive. In this article, we report that Pax6-elicited expression the human (h)RFPL1 in HeLa cells can be enhanced by vivo p53 binding to promoter therefore investigated hypothesis hRFPL1 regulates cell-cycle progression. Upon these cells, decreased cell number through kinase-dependent mechanism as PKC activates Cdc2 inhibits activity. antiproliferative led an increased population G(2)/M phase specific cyclin B1 downregulations, which were precluded proteasome inhibitor. Specifically, cytoplasm-localized prevented accumulation during interphase. Consequently, showed delayed entry into mitosis lengthening resulting from threefold increase G(2) duration. Given previous reports RFPL1 expressed differentiation, impact on provides novel insights