3-D Model of the bee venom acid phosphatase: insights into allergenicity.
作者： Dessislava Georgieva , Kerstin Greunke , Nicolay Genov , Christian Betzel
DOI: 10.1016/J.BBRC.2008.11.101
关键词:
摘要: Abstract The acid phosphatase Api m 3 is the major allergen of honeybee venom. Except for amino sequence, no other structural information enzyme available. We applied homology modeling to assign three-dimensional structure 3. homodimeric human prostatic was used model tertiary structure. IgE epitopes and antigenic sites were predicted using programs based on known analysis 3-D model. revealed an active site similar those histidine—type phosphatases with conservation catalytically important residues. observed substitutions in phosphate ion binding suggest differences substrate specificity comparison phosphatases. a very likely mechanism action.
cabdirect.org
elsevier.com
sci-hub.se 参考文章(32)
R L Van Etten, R Davidson, P E Stevis, H MacArthur, D L Moore, Covalent structure, disulfide bonding, and identification of reactive surface and active site residues of human prostatic acid phosphatase. Journal of Biological Chemistry. ,vol. 266, pp. 2313- 2319 ,(1991) , 10.1016/S0021-9258(18)52245-6
K Ostanin, E.H. Harms, P.E. Stevis, R Kuciel, M.M. Zhou, R.L. Van Etten, Overexpression, site-directed mutagenesis, and mechanism of Escherichia coli acid phosphatase. Journal of Biological Chemistry. ,vol. 267, pp. 22830- 22836 ,(1992) , 10.1016/S0021-9258(18)50022-3
G. Schneider, Y. Lindqvist, P. Vihko, Three-dimensional structure of rat acid phosphatase. The EMBO Journal. ,vol. 12, pp. 2609- 2615 ,(1993) , 10.1002/J.1460-2075.1993.TB05921.X
K S Porvari, P A Taavitsainen, R M Kurkela, G Schneider, Y Lindqvist, A M Herrala, P T Vihko, Site-directed mutagenesis of prostatic acid phosphatase. Catalytically important aspartic acid 258, substrate specificity, and oligomerization. Journal of Biological Chemistry. ,vol. 269, pp. 22642- 22646 ,(1994) , 10.1016/S0021-9258(17)31694-0
M. C. Peitsch, ProMod and Swiss-Model: Internet-based tools for automated comparative protein modelling Biochemical Society Transactions. ,vol. 24, pp. 274- 279 ,(1996) , 10.1042/BST0240274
R. A. Laskowski, M. W. MacArthur, D. S. Moss, J. M. Thornton, PROCHECK: a program to check the stereochemical quality of protein structures Journal of Applied Crystallography. ,vol. 26, pp. 283- 291 ,(1993) , 10.1107/S0021889892009944
Alison R. Hayman, Tartrate-resistant acid phosphatase (TRAP) and the osteoclast/immune cell dichotomy Autoimmunity. ,vol. 41, pp. 218- 223 ,(2008) , 10.1080/08916930701694667
T GRUNWALD, B BOCKISCH, E SPILLNER, J RING, R BREDEHORST, M OLLERT, Molecular cloning and expression in insect cells of honeybee venom allergen acid phosphatase (Api m 3). The Journal of Allergy and Clinical Immunology. ,vol. 117, pp. 848- 854 ,(2006) , 10.1016/J.JACI.2005.12.1331
E. Barboni, D.M. Kemeny, S. Campos, C.A. Vernon, The purification of acid phosphatase from honey bee venom (Apis mellifica) Toxicon. ,vol. 25, pp. 1097- 1103 ,(1987) , 10.1016/0041-0101(87)90266-2
B. Lee, F.M. Richards, The interpretation of protein structures: Estimation of static accessibility Journal of Molecular Biology. ,vol. 55, pp. 379- IN4 ,(1971) , 10.1016/0022-2836(71)90324-X