Manipulation of thyroid status and/or GH injection alters hepatic gene expression in the juvenile chicken.

摘要: Both thyroid hormone (T3) and growth (GH) are important regulators of somatic in birds mammals. Although T3-mediated gene transcription is well known, the molecular basis T3 interaction with GH on development remains unknown. In earlier studies, we discovered that exogenous alone increased accumulation visceral fat young chickens, while combination injections dietary worked synergistically to deplete body fat. present study, cDNA microarray quantitative RT-PCR analyses enabled us examine hepatic expression chickens after chronic manipulation status injection or T3. Thyroid modulates common unique sets genes involved a wide range functions (i.e., energy metabolism, storage transport, signal transduction, protein turnover drug detoxification). Hepatic 35 was altered by hypothyroidism (e.g., ADFP, ANGPTL3, GSTΑ, CAT, PPARG, HMGCL, GHR, IGF1, STAT3, THRSPα), whereas hyperthyroidism affected another cluster 13 IGFBP1, KHK, LDHB, BAIA2L1, SULT1B, TRIAD3). Several were identified which have not been previously ascribed as responsive DEFB9, EPS8L2, ARHGAP1, LASS2, INHBC). Exogenous 17 CCAR1, CYP2C45, GYS2, ENOB, HK1, FABP1, SQLE, SOCS2, UPG2). The T3+GH treatment depleted greatest amount fat, where 34 differentially expressed this group C/EBP, CDC42EP1, SYDE2, PCK2, PIK4CA, TH1L, GPT2, BHMT). marked reduction brought about synergism could involve modulation signaling via activity Ras superfamily switches, control diverse biological processes. conclusion, study provides first global analysis endocrine (T3 GH) regulation chicken.