Enrichment of Circular RNA Expression Deregulation at the Transition to Recurrent Spontaneous Seizures in Experimental Temporal Lobe Epilepsy.

摘要: Mesial temporal lobe epilepsy (mTLE) is a common form of and characterized by recurrent spontaneous seizures originating from the lobe. The majority mTLE patients develop pharmacoresistance to available anti-epileptic drugs (AEDs) while exhibiting severe pathological changes that can include hippocampal atrophy, neuronal death, gliosis chronic seizures. molecular mechanisms leading remain incompletely understood, but are known defects in post-transcriptional gene expression regulation, including non-coding RNAs (ncRNAs). Circular (circRNAs) class recently rediscovered ncRNAs with high levels brain proposed roles diverse processes. To explore potential role for circRNAs epilepsy, RNA-sequencing (RNA-seq) was performed on tissue rat perforant pathway stimulation (PPS) model TLE at different post-stimulation time points. This analysis revealed 218 differentially expressed (DE) circRNAs. Remarkably, these were changed occurrence first seizure (DOFS). pattern two circRNAs, circ_Arhgap4 circ_Nav3, further validated linked miR-6328 miR-10b-3p target respectively. study examine regulation during development epilepsy. It reveals an intriguing link between circRNA deregulation transition networks into state activity. Together, our results provide framework understanding mechanism-of-action TLE.