Protein expression profile related to cisplatin resistance in bladder cancer cell lines detected by two-dimensional gel electrophoresis.
作者： Yoshinori TAOKA , Kazumasa MATSUMOTO , Kazuya OHASHI , Satoru MINAMIDA , Masahiro HAGIWARA
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摘要: We used a proteomic approach to compare the differentially regulated protein expression profiles of cisplatin-naive and cisplatin-resistant bladder cancer cell lines screen candidate molecules related cisplatin resistance. The line T24 was established by stepwise exposure cells up 40 μM cisplatin. performed comprehensive study in that included (T24) (T24CDDPR) means agarose two-dimensional gel electrophoresis followed analysis liquid chromatography tandem mass spectroscopy. identified 25 obviously different spots for CDDPR. Seven had increased 18 decreased T24CDDPR compared those T24. Cytoskeletal proteins enzyme modulators were prominent among differential proteins. Of proteins, we selected HNRNPA3, PCK2, PPL, PGK1, TKT, SERPINB2, GOT2, EIF3A further validation Western blot. SERPINB2 more than 1.5-times incremental PCK2 PPL expressions less 20% results new this could be valuable lead development molecular marker.
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