Detection of tumor-associated antigens in culture supernatants using autoantibodiesin sera from patients with bladder cancer
作者： Sho MINAMI , Ryo NAGASHIO , Junpei UEDA , Kazumasa MATSUMOTO , Naoki GOSHIMA
关键词:
摘要: Secreted proteins play essential roles in the process of tumorigenesis, and analysis tumor- secreted has been suggested as a promising strategy for identifying cancer biomarkers. In this study, we performed proteomic to identify from bladder cell lines that are recognized by autoantibodies sera patients with cancer. addition, against identified were validated using dot-blot array normal controls. As results, detected twenty-five thir - ty-two immunoreactive spots high- low-grade cancer, re- spectively. validation revealed serum IgG levels anti-calreticulin matrix metalloproteinase-2 (MMP2) significantly higher pa- tients than controls (both P < 0.05). Furthermore, level anti-MMP2 autoantibody was compared (P On multivariate analysis, an in- dependent predictor cancer-specific survival ( Based on these findings, lev els MMP2 may be novel biomarker candidates its clinical outcome.
sci-hub.se 参考文章(50)
A. Pekáriková, D. Sánchez, L. Palová-Jelínková, M. Šimšová, Z. Beneš, I. Hoffmanová, P. Drastich, I. Janatková, T. Mothes, H. Tlaskalová-Hogenová, L. Tučková, Calreticulin is a B cell molecular target in some gastrointestinal malignancies Clinical and Experimental Immunology. ,vol. 160, pp. 215- 222 ,(2009) , 10.1111/J.1365-2249.2009.04085.X
Roger Moe, Mary L. Disis, Ann E. Murphy, Robert B. Livingston, Margit Jeschke, Elaine McGlynn, Wei Chen, Martin A. Cheever, Emanuel Calenoff, Graham McLaughlin, Nick Lydon, Bernd Groner, Existent T-cell and antibody immunity to HER-2/neu protein in patients with breast cancer. Cancer Research. ,vol. 54, pp. 16- 20 ,(1994)
M. Onisto, M. Micela, C. Caenazzo, S. Garbisa, W. G. Stetler-Stevenson, L. Masiero, C. Di Francesco, G. Scagliotti, L. A. Liotta, Correlation of serum metalloproteinase levels with lung cancer metastasis and response to therapy. Cancer Research. ,vol. 52, pp. 4548- 4549 ,(1992)
Susumu Kageyama, Takahiro Isono, Shinjiro Matsuda, Yoshihiro Ushio, Shinji Satomura, Akito Terai, Yoichi Arai, Mutsushi Kawakita, Yusaku Okada, Tatsuhiro Yoshiki, Urinary calreticulin in the diagnosis of bladder urothelial carcinoma International Journal of Urology. ,vol. 16, pp. 481- 486 ,(2009) , 10.1111/J.1442-2042.2009.02287.X
F FERNANDEZMADRID, Autoantibodies in breast cancer sera: candidate biomarkers and reporters of tumorigenesis. Cancer Letters. ,vol. 230, pp. 187- 198 ,(2005) , 10.1016/J.CANLET.2004.12.017
Eunsil Yu, Inchul Lee, Ghil-Suk Yoon, Kyuyoung Song, Yusun Jung, Hee-Bom Moon, Hojung Lee, Nuclear Matrix of Calreticulin in Hepatocellular Carcinoma Cancer Research. ,vol. 60, pp. 1117- 1120 ,(2000)
Luca Bini, Barbara Magi, Barbara Marzocchi, Felice Arcuri, Sergio Tripodi, Marcella Cintorino, Jean-Charles Sanchez, SÉVerine Frutiger, Graham Hughes, Vitaliano Pallini, Denis F. Hochstrasser, Piero Tosi, Protein expression profiles in human breast ductal carcinoma and histologically normal tissue Electrophoresis. ,vol. 18, pp. 2832- 2841 ,(1997) , 10.1002/ELPS.1150181519
S P Monig, S E Baldus, J K Hennecken, D B Spiecker, G Grass, P M Schneider, J Thiele, H P Dienes, A H Holscher, Expression of MMP-2 is associated with progression and lymph node metastasis of gastric carcinoma Histopathology. ,vol. 39, pp. 597- 602 ,(2001) , 10.1046/J.1365-2559.2001.01306.X
Eleftherios P Diamandis, How Are We Going to Discover New Cancer Biomarkers? A Proteomic Approach for Bladder Cancer Clinical Chemistry. ,vol. 50, pp. 793- 795 ,(2004) , 10.1373/CLINCHEM.2004.032177
Chiung-Nien Chen, Cheng-Chi Chang, Ting-En Su, Wen-Ming Hsu, Yung-Ming Jeng, Ming-Chih Ho, Fon-Jou Hsieh, Po-Huang Lee, Min-Liang Kuo, Hsinyu Lee, King-Jen Chang, Identification of Calreticulin as a Prognosis Marker and Angiogenic Regulator in Human Gastric Cancer Annals of Surgical Oncology. ,vol. 16, pp. 524- 533 ,(2009) , 10.1245/S10434-008-0243-1