Advanced Methodologies in High-Throughput Sequencing of Immune Repertoires
作者： Simon Friedensohn , Tarik A. Khan , Sai T. Reddy
DOI: 10.1016/J.TIBTECH.2016.09.010
关键词:
摘要: In recent years, major efforts have been made to develop sophisticated experimental and bioinformatic workflows for sequencing adaptive immune repertoires. The immunological insight gained has applied fields as varied lymphocyte biology, immunodiagnostics, vaccines, cancer immunotherapy, antibody engineering. this review, we provide a detailed overview of these advanced methodologies, focusing specifically on strategies reduce errors bias achieve high-throughput pairing variable regions (e.g., heavy-light or alpha-beta chains). addition, highlight technologies single-cell transcriptome that can be integrated with Finally, perspective repertoire its ability impact basic immunology, biopharmaceutical drug discovery development, immunotherapy.
sci-hub.se 参考文章(80)
Francois Vigneault, Adrian Wrangham Briggs, Single cell bar-coding for antibody discovery ,(2014)
Yariv Wine, Andrew P Horton, Gregory C Ippolito, George Georgiou, Serology in the 21st century: the molecular-level analysis of the serum antibody repertoire Current Opinion in Immunology. ,vol. 35, pp. 89- 97 ,(2015) , 10.1016/J.COI.2015.06.009
Victor Greiff, Pooja Bhat, Skylar C. Cook, Ulrike Menzel, Wenjing Kang, Sai T. Reddy, A bioinformatic framework for immune repertoire diversity profiling enables detection of immunological status Genome Medicine. ,vol. 7, pp. 49- 49 ,(2015) , 10.1186/S13073-015-0169-8
Kenneth Murphy, Casey Weaver, Janeway's immunobiology ,(2007)
Dmitry A. Bolotin, Ilgar Z. Mamedov, Olga V. Britanova, Ivan V. Zvyagin, Dmitriy Shagin, Svetlana V. Ustyugova, Maria A. Turchaninova, Sergey Lukyanov, Yury B. Lebedev, Dmitriy M. Chudakov, NEXT GENERATION SEQUENCING FOR TCR REPERTOIRE PROFILING: PLATFORM-SPECIFIC FEATURES AND CORRECTION ALGORITHMS European Journal of Immunology. ,vol. 42, pp. 3073- 3083 ,(2012) , 10.1002/EJI.201242517
Evgeny S Egorov, Ekaterina M Merzlyak, Andrew A Shelenkov, Olga V Britanova, George V Sharonov, Dmitriy B Staroverov, Dmitriy A Bolotin, Alexey N Davydov, Ekaterina Barsova, Yuriy B Lebedev, Mikhail Shugay, Dmitriy M Chudakov, None, Quantitative Profiling of Immune Repertoires for Minor Lymphocyte Counts Using Unique Molecular Identifiers Journal of Immunology. ,vol. 194, pp. 6155- 6163 ,(2015) , 10.4049/JIMMUNOL.1500215
Aleksandra A. Kolodziejczyk, Jong Kyoung Kim, Valentine Svensson, John C. Marioni, Sarah A. Teichmann, The technology and biology of single-cell RNA sequencing. Molecular Cell. ,vol. 58, pp. 610- 620 ,(2015) , 10.1016/J.MOLCEL.2015.04.005
Maria A Turchaninova, Olga V Britanova, Dmitriy A Bolotin, Mikhail Shugay, Ekaterina V Putintseva, Dmitriy B Staroverov, George Sharonov, Dmitriy Shcherbo, Ivan V Zvyagin, Ilgar Z Mamedov, Carsten Linnemann, Ton N Schumacher, Dmitriy M Chudakov, None, Pairing of T-cell receptor chains via emulsion PCR European Journal of Immunology. ,vol. 43, pp. 2507- 2515 ,(2013) , 10.1002/EJI.201343453
Christian E. Busse, Irina Czogiel, Peter Braun, Peter F. Arndt, Hedda Wardemann, Single-cell based high-throughput sequencing of full-length immunoglobulin heavy and light chain genes European Journal of Immunology. ,vol. 44, pp. 597- 603 ,(2014) , 10.1002/EJI.201343917
Máire F. Quigley, Jorge R. Almeida, David A. Price, Daniel C. Douek, Unbiased molecular analysis of T cell receptor expression using template-switch anchored RT-PCR. Current protocols in immunology. ,vol. 94, ,(2011) , 10.1002/0471142735.IM1033S94