Division of labour between Myc and G1 cyclins in cell cycle commitment and pace control

摘要: A body of evidence has shown that the control E2F transcription factor activity is critical for determining cell cycle entry and proliferation. However, an understanding precise determinants this control, including role other cell-cycle regulatory activities, not been clearly defined. Here, recognizing contributions individual components could be masked by heterogeneity in populations cells, we model potential roles together with use integrated system to follow dynamics at single-cell level real time. These analyses reveal crossing a threshold amplitude accumulation determines commitment. Importantly, find Myc modulating amplitude, whereas cyclin D/E activities have little effect on but do contribute modulation duration activation, thereby affecting pace progression.