Novel nitrile hydratase

作者: Toshifumi Yamaki , Shinichi Banba , Toshihiro Oikawa , Hideki Kobayashi , Eishi Tanaka

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摘要: It is aimed at providing the amino acid sequence of a nitrile hydratase having novel mutation point and base gene. also method modifying an enzyme activity. As means achieving above objects, mutant, which obtained by transferring into Pseudonocardia thermophila JCM3095-origin consisting two types hetero subunits, gene are provided. Further, modified specifying region to be in stereostructure and/or altering (substituting, inserting, deleting, etc.) acids corresponding residues constituting region. Thus, compound can efficiently converted amide compound, compared with existing techniques.

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