Influence of substratum surface chemistry/energy and topography on the human fetal osteoblastic cell line hFOB 1.19: Phenotypic and genotypic responses observed in vitro ☆

摘要: Time-dependent phenotypic response of a model osteoblast cell line (hFOB 1.19, ATCC, and CRL-11372) to substrata with varying surface chemistry topography is reviewed within the context extant cell-adhesion theory. Cell-attachment proliferation kinetics are compared using morphology as leading indicator phenotype. Expression (alpha2, alpha3, alpha4, alpha5, alphav, beta1, beta3) integrins, vinculin, well secretion osteopontin (OP) type I collagen (Col I) supplement this visual assessment hFOB growth. It concluded that significant events-contact, attachment, spreading, proliferation-are similar on all surfaces, independent substratum chemistry/energy. However, sequence events significantly delayed attenuated hydrophobic (poorly water-wettable) surfaces exhibiting characteristically low-attachment efficiency long induction periods before cells engage in an exponential-growth phase. Results suggest 'time-cell-substratum-compatibility-superposition principle' at work wherein bioadhesive outcomes can be ultimately achieved types hydrophilicity, but time required arrive outcome increases decreasing cell-substratum-compatibility. Genomic proteomic tools offer unprecedented opportunity directly measure changes cellular machinery lead observed responses different materials. But for purpose measuring structure-property relationships guide biomaterial development, genomic/proteomic should applied early adhesion/spreading process have remodel cell-substratum interface, effectively erasing cause effect between cell-substratum-compatibility properties. IMPACT STATEMENT: This review quantifies among phenotype, chemistry/energy, topography, contact revealing most useful pursuit understanding adhesion if process.