miR-1238 inhibits cell proliferation by targeting LHX2 in non-small cell lung cancer.

摘要: // Xiangguang Shi 1,2,* , Lei Zhan Can Xiao 3,* Zhe 1,2 Haiping Yang Longqiang Wang Jun Zhao 2,3 and Hong-Tao Zhang 1 Soochow University Laboratory of Cancer Molecular Genetics, Medical College University, Suzhou, China 2 Suzhou Key for 3 The First Affiliated Hospital * These authors have contributed equally to this work Correspondence to: Zhang, email: Keywords : NSCLC, miR-1238, LHX2, cell proliferation Received January 30, 2015 Accepted May 13, Published 22, Abstract In human cancers, dysregulated expression LIM-homeobox gene (LHX2) downregulation miR-1238 has been reported separately. However, the relationship between them remains unclear. We investigated functional contribution regulation LHX2 in non-small lung cancer (NSCLC). Here, computational algorithms predicted that 3’-untranslated region (3’-UTR) is a target miR-1238. Luciferase assays validated directly bound 3’-UTR . qRT-PCR western blot analyses further confirmed overexpression mimic NSCLC A549 LTEP-α-2 cells inhibited endogenous mRNA protein. Moreover, ectopic suppressed cellular viability proliferation. siRNA-induced knockdown copied phenotype cycle. addition, was frequently decreased tissues reversely correlated with expression, which increased tissues. Collectively, our findings demonstrate inhibit at least partly via repression shedding light on mechanistic interaction carcinogenesis. Furthermore, data suggest could be promising therapeutic strategy treatment.