Growth of the white rust fungus Albugo candida in callus tissue of Brassica juncea

摘要: Leaf and stem explants of Brassica juncea cv. Pusa Bold produced callus tissue plantlets on Murashige Skoog medium at 18 °C. Among the various organs inflorescences, systemically infected with Albugo candida from hypertrophied peduncles thickened terminal leaves proved suitable for production dua cultures biotroph its host, success former being better than latter. Dual were also established ovaries, but poorly. Infected grew faster healthy counterpart. sten pieces rust pustules did not form callus. The dual culture showed abundant coenocytic intercellular mycelium spherical haustoria within host cells. maintained 15 20 °C to 30 d only sporangial chains that remained confined tissue, whereas 25 ° contained both sporangia oospores, like those A. found in naturally tissues. pathoge could be prolonged periods by periodic subculturing it kept pace growth tissue. Placement an close contact a one no result infection