microRNAs associated with early neural crest development in Xenopus laevis.

摘要: The neural crest (NC) is a class of transitory stem cell-like cells unique to vertebrate embryos. NC arise within the dorsal tube where they undergo an epithelial mesenchymal transition in order migrate and differentiate throughout developing embryo. derivative cell types give rise multiple tissues, including craniofacial skeleton, peripheral nervous system skin pigment cells. Several well-studied gene regulatory networks underpin development, which when disrupted can lead various neurocristopathies such as craniofrontonasal dysplasia, DiGeorge syndrome some forms cancer. Small RNAs, microRNAs (miRNAs) are non-coding RNA molecules important post-transcriptional silencing critical for cellular regulation expression. To uncover novel small RNAs development we used high definition adapters next generation sequencing libraries derived from ectodermal explants Xenopus laevis embryos induced form tissue. Ectodermal blastula animal pole (blastula) stage tissues were also sequenced. We show that miR-427 highly abundant all four tissue though isoform specific manner define set 11 miRNAs enriched NC. In addition, miR-301a miR-338 expressed both suggesting role these maintaining phenotype have characterised embryonic treated ectoderm, or This has identified highlighted differential expression isoforms.