Induction of skeletal abnormalities and autophagy in Paracentrotus lividus sea urchin embryos exposed to gadolinium.
作者： Chiara Martino , Roberto Chiarelli , Liana Bosco , Maria Carmela Roccheri
DOI: 10.1016/J.MARENVRES.2017.07.007
关键词:
摘要: Gadolinium (Gd) concentration is constantly increasing in the aquatic environment, becoming an emergent environmental pollutant. We investigated effects of Gd on Paracentrotus lividus sea urchin embryos, focusing skeletogenesis and autophagy. observed a delay biomineral deposition at 24 hours post fertilization (hpf), strong impairment skeleton growth 48 hpf, frequently displayed by asymmetrical pattern. Skeleton was found partially resumed recovery experiments. The mesodermal cells designated to biomineralization were correctly migrated 24 but not hpf. Western blot analysis showed increase LC3-II autophagic marker Confocal microscopy studies confirmed increased number autophagolysosomes autophagosomes. Results show hazard marine indicating that able affect different aspects development: morphogenesis, biomineralization, stress response through
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