Sequence, expression, and chromosomal assignment of a human sperm outer dense fiber gene

摘要: Outer dense fibers (ODFs) are located on the outside of axoneme in midpiece and principal piece mammalian sperm tail may help to maintain passive elastic structures recoil tail. We have identified describe here a human gene that is homologous Mst(3)CGP family Drosophila melanogaster encodes an ODF protein 241 amino acids. The transcribed region has size approximately 1 kb contains two exons 416 bp 406 bp, respectively, not including 3' untranslated region. expressed testis but spleen, kidney, or brain resembles this respect expression male germline. An antiserum raised against synthetic peptide derived from N-terminus encoded sequence 32 kDa extract flagella. By Southern-blot analyses situ hybridization, was localized band q22 chromosome 8. isolation encoding provide ability identify investigate, molecular level, possible reasons for infertility dependent flagellar disturbances.