Comparison of Y-chromosome-linked TSPY, TSPY2, and PRAMEY genes in Taurus cattle, yaks, and interspecific hybrid bulls.

摘要: Domestic yaks (Bos grunniens) and domestic Taurus cattle taurus) are closely related. An interesting phenomenon in interspecific crossings is male sterility the F1 hybrid (yattle) F2 backcross, with no late meiotic cells or spermatids seminiferous tubules. The mammalian Y chromosome crucial for spermatogenesis fertility. This study investigated copy number variations mRNA of Y-transitional region genes TSPY2 (testis specific protein, Y-linked 2 testis-specific Y-encoded protein 3-like) PRAMEY (preferentially expressed antigen melanoma, Y-linked), Y-ampliconic TSPY (testis-specific 1-like), ZNF280BY (zinc finger 280B, Y-linked) HSFY (heat-shock transcription factor, mature testes from cattle, yaks, yattle. Phylogenetic trees divided 33 copies into major types (TSPY-T1 TSPY-T2), 19 (TSPY2-T1 T2), 8 4 (PRAMEY-T1 to T4). Searching by Basic Local Alignment Search Tool coding sequences GenBank revealed that was conserved Bovidae. TSPY2-T2 were absent, whereas PRAMEY-T2 PRAMEY-T4 amplified on yak chromosome. average numbers TSPY-T2 significantly different between yaks. TSPY-T2, TSPY2, PRAMEY, ZNF280BY, uniquely predominantly testes. Reverse-transcription quantitative PCR showed PRAMEY-T2, HSFY, protamine 1 (PRM1), (PRM2) almost not PRM1 PRM2 used as positive markers spermatozoa. Thus, our results genomic structure differed Dysregulated expression HSPY, gene may be associated