Cellular and functional specificity among ferritin-like proteins in the multicellular cyanobacterium Nostoc punctiforme.

摘要: Ferritin-like proteins constitute a remarkably heterogeneous protein family, including ferritins, bacterioferritins and Dps proteins. The genome of the filamentous heterocyst-forming cyanobacterium Nostoc punctiforme encodes five ferritin-like In present paper, we report multidimensional characterization these Our phylogenetic bioinformatics analyses suggest both structural physiological differences among expression genes responded differently to hydrogen peroxide treatment, with significantly higher rise in transcript level for Npun_F3730 as compared other four genes. A specific role cells tolerance against was also supported by inactivation Npun_F3730, Npun_R5701 Npun_R6212; these, only ΔNpun_F3730 strain showed an increased sensitivity wild type. Analysis promoter-GFP reporter fusions indicated that were expressed all cell types diazotrophic culture, while Npun_F6212 specifically heterocysts. study provides first comprehensive analysis combining functional differentiation cellular specificity within this important group multicellular cyanobacterium.