Notas Científicas Transformação genética de laranja 'Valência' com o gene cecropin MB39

作者： Ricardo Harakava , Luis Gustavo de Paoli , Beatriz Madalena , Januzzi Mendes , Raquel Luciana Boscariol Camargo

DOI:

关键词:

摘要: The objective of this work was to produce 'Valencia' sweet orange transgenic plants with the cecropin MB39 gene controlled by a phenylalanine ammonia-lyase promoter from citrus in order direct expression xylem vessels. genetic transformation mediated Agrobacterium tumefaciens co-culture epicotyl segments. Eleven were selected PCR amplification 189 bp fragment, which acclimatized greenhouse. integration transgene confirmed three Southern blot analysis.

暂无可下载资源，当前可以选择系统获取到有开放资源时通知我或者直接发起求助文献求助

参考文章(10)

Maria L. Ishida, Peter C. Andersen, Breno Leite, Effect of Vitis vinifera L. cv. Chardonnay xylem fluid on cecropin B activity against Xylella fastidiosa Physiological and Molecular Plant Pathology. ,vol. 64, pp. 73- 81 ,(2004) , 10.1016/J.PMPP.2004.07.001

P. C. Andersen, B. Leite, M. T. Momol, E. A. Momol, M. L. Ishida, B. V. Brodbeck, Influence of Vitis xylem fluid and xylem fluid plus cecropin on growth of Xylella fastidiosa Vitis: Journal of Grapevine Research. ,vol. 43, pp. 19- 25 ,(2015)

Lowell D. Owens, Thomas M. Heutte, A Single Amino Acid Substitution in the Antimicrobial Defense Protein Cecropin B Is Associated with Diminished Degradation by Leaf Intercellular Fluid Molecular Plant-microbe Interactions. ,vol. 10, pp. 525- 528 ,(1997) , 10.1094/MPMI.1997.10.4.525

Weliton Antonio Bastos de Almeida, Francisco de Assis Alves Mourão Filho, Beatriz Madalena Januzzi Mendes, Alexandra Pavan, Adriana Pinheiro Martinelli Rodriguez, Agrobacterium-mediated transformation of Citrus sinensis and Citrus limonia epicotyl segments Scientia Agricola. ,vol. 60, pp. 23- 29 ,(2003) , 10.1590/S0103-90162003000100005

C�sar Petri, Lorenzo Burgos, Transformation of fruit trees. Useful breeding tool or continued future prospect Transgenic Research. ,vol. 14, pp. 15- 26 ,(2005) , 10.1007/S11248-004-2770-2

Jesse M. Jaynes, Pablito Nagpala, Luis Destéfano-Beltrán, Jian Hong Huang, JaeHo Kim, Timothy Denny, Selim Cetiner, Expression of a Cecropin B lytic peptide analog in transgenic tobacco confers enhanced resistance to bacterial wilt caused by Pseudomonas solanacearum Plant Science. ,vol. 89, pp. 43- 53 ,(1993) , 10.1016/0168-9452(93)90169-Z

Michael Zasloff, Antimicrobial peptides of multicellular organisms Nature. ,vol. 415, pp. 389- 395 ,(2002) , 10.1038/415389A

Håkan Steiner, David Andreu, R.B. Merrifield, Binding and action of cecropin and cecropin analogues: antibacterial peptides from insects. Biochimica et Biophysica Acta. ,vol. 939, pp. 260- 266 ,(1988) , 10.1016/0005-2736(88)90069-7

J. J. Doyle, Isolation of plant DNA from fresh tissue Focus. ,vol. 12, pp. 13- 15 ,(1990)

10.

Fernando Alves de Azevedo, Francisco de Assis Alves Mourão Filho, Evandro Henrique Schinor, Luis Gustavo de Paoli, Beatriz Madalena Januzzi Mendes, Ricardo Harakava, Dean W. Gabriel, Richard F. Lee, GUS gene expression driven by a citrus promoter in transgenic tobacco and 'Valencia' sweet orange Pesquisa Agropecuaria Brasileira. ,vol. 41, pp. 1623- 1628 ,(2006) , 10.1590/S0100-204X2006001100008

Notas Científicas Transformação genética de laranja 'Valência' com o gene cecropin MB39

来源期刊

我的账户

Notas Científicas Transformação genética de laranja 'Valência' com o gene cecropin MB39

来源期刊

相似文章 0

我的账户