Early attachment of uncultured retinal pigment epithelium from aged donors onto Bruch's membrane explants.

摘要: Retinal pigment epithelium (RPE) transplantation might replace cells lost as a consequence of choroidal neovascular membrane excision in patients with age-related macular degeneration (AMD). Autologous RPE harvested from peripheral biopsy may overcome problems immune rejection. To study the feasibility autologous cell transplantation, authors examined attachment freshly aged donors onto Bruch's explants, debrided to (1) remove or (2) preserve basement membrane. Human retinal epithelial sheets were adult donor eyes (N = 12, mean age 79.00 +/- 9.40 years) and, following incubation collagenase, mechanically fragmented into microaggregates. Microaggregates (approximately 120 000 cells) seeded paired explants (7 mm diameter) and incubated for 20 min, 1, 4, 24 hr at 37 degrees C. The percent coverage surface by microaggregates was determined sampling center scanning electron microscopy. microaggregate significantly greater all time points analysed samples intact versus those an exposed inner collagenous layer. Coverage debridements retaining 1.83 1.10% 3.54 2.14% 1 hr, 8.68 2.63% 4 hr. lacking 0.10 0.04% 0.39 0.25% 0.63 0.42% Based on their morphologic appearance, many dying early seeding. increase coverage, four times above number specimens showed no attached There significant approximately three-fold presence These results indicate that if human are used some modification must be considered eventual survival.