A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR

摘要: A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using immuno-PCR has been developed. The sensitivity the detection nonstructural protein 1 (NS 1) antigenically common among viruses was 10(1.0-2.0) times higher than that RT-PCR genome. For hemagglutinin (HA) subtype-specific antigen, this assay anti-HA monoclonal antibodies attained a up to 10(7.0-8.0) those virus isolation or RT-PCR.