miRNA-650 exerts anti-leukemia activity by inhibiting cell proliferation through Gfi1 targeting.

摘要: Background: Acute myeloid leukemia (AML) is the most common malignancy of bone marrow with a high mortality. Recent advances in high-throughput sequencing have led to identification various miRNAs implicated pathogenesis AML. We found this study that miR-650, miRNA was traditionally considered participate onset hepatocellular carcinoma, might play significant role AML development and progression. Methods: qRT-PCR used detect expression miR-650 Gfi1 patients healthy controls. Next, luciferase assay conducted verify target effect on Gfi1. Moreover, CCK-8 performed evaluate proliferation cells presence absence Results: downregulated whereas upregulated. could negatively regulate via direct targeting its 3'-UTR, which confirmed by assay. In addition, overexpression reduced cell K562 cells, an increase observed when were transfected inhibitor, compromised response knockdown Conclusions: Our research demonstrated modulates through affecting Gfi1, occurs action.