Heat flux as an on-line indicator of metabolic activity in pilot scale bioreactor during the production of Bacillus thuringiensis var. galleriae-based biopesticides
作者： R.K.I Anderson , Kunthala Jayaraman , D Voisard , Ian W Marison , U.von Stockar
DOI: 10.1016/S0040-6031(01)00709-2
关键词:
摘要: The effective biotechnological process development depends on the system for monitoring activity of culture during cultivation. feasibility metabolic flux cultivation Bacillus thuringiensis var. galleriae (Btg) in pilot scale bioreactor was studied. All possible sources heat generation and transfer were accounted to determine caused by alone. Successful demonstration measurements has revealed scale-up characteristics microbial based data. In addition, influence complex media flux, sporulation insecticidal crystal protein (ICP) synthesis understood. Nature necessitated fed-batch with glucose solution feeding proportions production rate subsequent optimization concentration had resulted an enhanced ICP concentration. As a significant outcome, into cooling water maintenance temperature found be definite correlation rate. This allowed opportunity understanding status continuous on-line profile industrial fermentation cost effectiveness. Continuous assessment cultures through could exploited enhancing desired process(s) fermentation.
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