Clostridium orbiscindens sp. nov. a Human Intestinal Bacterium Capable of Cleaving the Flavonoid C-Ring
作者： J. Winter , M. R. Popoff , P. Grimont , V. D. Bokkenheuser
DOI: 10.1099/00207713-41-3-355
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摘要: Clostridium orbiscindens sp. nov. is an obligate anaerobe that capable of cleaving the C-3-C-4 bond natural anticarcinogen quercetin. The metabolic products, 3,4-dihydroxyphenylacetic acid and presumably phlorglucinol, are not known to possess properties. This organism was isolated from human feces. On sheep blood agar plates C. forms minute, irregular, convex, gray or white, shiny, smooth, nonhemolytic colonies. It beta-hemolytic on rabbit agar. motile peritrichous rods gram variable. Subpolar spores common. Cultures resistant 80°C for 10 min. Capsules absent. asaccharolytic does metabolize esculin, urea, meat, gelatin, casein, nitrate. G+C content 56 57 mol%. DNA hybridization experiments did reveal relatedness phenotypically similar strains. Strain 265 (= ATCC 49531) type strain.
sgmjournals.org参考文章(14)
Reinhard HollÄnder, Sabine Pohl, Deoxyribonucleic acid base composition of bacteria. Zentralblatt für Bakteriologie. 1. Abt. Originale A, Medizinische Mikrobiologie, Infektionskrankheiten und Parasitologie. ,vol. 246, pp. 236- 275 ,(1980) , 10.1016/S0172-5599(80)80117-9
J Winter, L H Moore, V R Dowell, V D Bokkenheuser, C-ring cleavage of flavonoids by human intestinal bacteria. Applied and Environmental Microbiology. ,vol. 55, pp. 1203- 1208 ,(1989) , 10.1128/AEM.55.5.1203-1208.1989
Michael N. Gould, Jeffrey A. Johnson, Ajit K. Verma, Martin A. Tanner, Inhibition of 7,12-Dimethylbenz(a)anthracene- and N-Nitrosomethylurea-induced Rat Mammary Cancer by Dietary Flavonol Quercetin Cancer Research. ,vol. 48, pp. 5754- 5758 ,(1988)
Patrick A. D. Grimont, Michel Y. Popoff, Francine Grimont, Colette Coynault, Muguette Lemelin, Reproducibility and correlation study of three deoxyribonucleic acid hybridization procedures Current Microbiology. ,vol. 4, pp. 325- 330 ,(1980) , 10.1007/BF02605371
V. D. Bokkenheuser, G. N. Morris, A. E. Ritchie, L. V. Holdeman, J. Winter, Biosynthesis of Androgen from Cortisol by a Species of Clostridium Recovered From Human Fecal Flora The Journal of Infectious Diseases. ,vol. 149, pp. 489- 494 ,(1984) , 10.1093/INFDIS/149.4.489
Klaus Goerttler, Heinz Loehrke, Inhibition of the prenatal dimethylbenz[a]anthracene-induced tumour initiation in mice by prior administration of 7,8-benzoflavone. Carcinogenesis. ,vol. 7, pp. 1187- 1189 ,(1986) , 10.1093/CARCIN/7.7.1187
T. D. Wilkins, T. Thiel, Modified Broth-Disk Method for Testing the Antibiotic Susceptibility of Anaerobic Bacteria Antimicrobial Agents and Chemotherapy. ,vol. 3, pp. 350- 356 ,(1973) , 10.1128/AAC.3.3.350
L. G. Wayne, W. E. C. Moore, E. Stackebrandt, O. Kandler, R. R. Colwell, M. I. Krichevsky, H. G. Truper, R. G. E. Murray, P. A. D. Grimont, D. J. Brenner, M. P. Starr, L. H. Moore, Report of the Ad Hoc Committee on Reconciliation of Approaches to Bacterial Systematics International Journal of Systematic and Evolutionary Microbiology. ,vol. 37, pp. 463- 464 ,(1987) , 10.1099/00207713-37-4-463
Jorge H. Crosa, Don J. Brenner, Stanley Falkow, Use of a single-strand specific nuclease for analysis of bacterial and plasmid deoxyribonucleic acid homo- and heteroduplexes. Journal of Bacteriology. ,vol. 115, pp. 904- 911 ,(1973) , 10.1128/JB.115.3.904-911.1973
R. J. Owen, L. R. Hill, S. P. Lapage, Determination of DNA base compositions from melting profiles in dilute buffers. Biopolymers. ,vol. 7, pp. 503- 516 ,(1969) , 10.1002/BIP.1969.360070408