EPR of Cobalt-Substituted Zinc Enzymes

摘要: Co(II) is sometimes utilized as a spectroscopically active substitute for Zn(II) in enzymes. Metal binding sites enzymes that contain catalytically generally yield high-spin S = 3/2 ions when substituted with cobalt, and these provide EPR spectra rich information. Extracting this information involves an appreciation of the extent to which properties mirror those Zn(II), careful sample preparation biochemical characterization, recording data, ability interpret quantitative way. Here, applicability structural functional mimic considered brief update studies literature presented. Methods substitution by are described. Recording will useful not trivial exercise, experimental considerations discussed some detail. The analysis terms spin-Hamiltonian parameters described, along their interpretation terms. Complementary techniques very briefly discussed, case study presented example how can mechanistic on zinc enzyme unavailable other techniques.