Identification and evaluation of an infertility-associated ZP3 epitope from the marsupial brushtail possum (Trichosurus vulpecula).

作者: Xianlan Cui , Janine A. Duckworth , Frank C. Molinia , Phil E. Cowan

DOI: 10.1016/J.VACCINE.2009.11.052

关键词:

摘要: Immunologically based fertility control vaccines against zona pellucida (ZP) proteins are being developed in New Zealand for biocontrol of the brushtail possum (Trichosurus vulpecula), an introduced Australian marsupial pest. We have shown that immunization female possums with recombinant ZP3 protein (rZP3) reduced by 79%. To enhance specificity immunocontraceptive vaccines, B-cell epitopes on were mapped using sera immunized rZP3 and subjected to a trial. The amino acid sequence full-length was used synthesize complete set 83 (12-mer) biotinylated peptides each overlap five acids neighboring peptides. modified enzyme-linked immunosorbent assay (ELISA) identify continuous recognized antibodies rZP3. Sixteen identified protein. Comparison ELISA binding patterns these individual status rZP3-immunized only one epitope (amino 156-172) be associated infertility. However, this showed no significant reduction fertility. possible reasons failure potential infertility discussed.

参考文章(57)
J. A. Duckworth, A. E. Byrom, P. Fisher, C. Horn, Pest Control: Does the Answer Lie in New Biotechnologies? Springer Berlin Heidelberg. pp. 421- 434 ,(2006) , 10.1007/3-540-30023-6_27
Cheryl S. Asa, Ingrid J. Porton, Wildlife contraception : issues, methods, and applications Johns Hopkins University Press. ,(2005)
Ya-Huan Lou, Kwan-Kyu Park, Sally Agersborg, Pascale Alard, Kenneth S. K. Tung, Retargeting T Cell-Mediated Inflammation: A New Perspective on Autoantibody Action Journal of Immunology. ,vol. 164, pp. 5251- 5257 ,(2000) , 10.4049/JIMMUNOL.164.10.5251
M. Hosaka, M. Nagahama, W.S. Kim, T. Watanabe, K. Hatsuzawa, J. Ikemizu, K. Murakami, K. Nakayama, Arg-X-Lys/Arg-Arg motif as a signal for precursor cleavage catalyzed by furin within the constitutive secretory pathway. Journal of Biological Chemistry. ,vol. 266, pp. 12127- 12130 ,(1991) , 10.1016/S0021-9258(18)98867-8
Kimberly M. Frank, Robin O. Lyda, Jay F. Kirkpatrick, Immunocontraception of captive exotic species Zoo Biology. ,vol. 24, pp. 349- 358 ,(2005) , 10.1002/ZOO.20060
Renuka Kaul, Neela Sivapurapu, Abhijit Afzalpurkar, V. Srikanth, Chhabi K. Govind, Satish K. Gupta, Immunocontraceptive potential of recombinant bonnet monkey (Macaca radiata) zona pellucida glycoprotein-C expressed in Escherichia coli and its corresponding synthetic peptide Reproductive Biomedicine Online. ,vol. 2, pp. 33- 39 ,(2001) , 10.1016/S1472-6483(10)62186-4
Kenneth S.K. Tung, Jennifer Ang, Yahuan Lou, ZP3 peptide vaccine that induces antibody and reversible infertility without autoimmune oophoritis. American Journal of Reproductive Immunology. ,vol. 35, pp. 181- 183 ,(1996) , 10.1111/J.1600-0897.1996.TB00028.X
SK Gupta, Neelu Srivastava, Sangeeta Choudhury, Archana Rath, Neela Sivapurapu, GK Gahlay, Deepika Batra, None, Update on zona pellucida glycoproteins based contraceptive vaccine. Journal of Reproductive Immunology. ,vol. 62, pp. 79- 89 ,(2004) , 10.1016/J.JRI.2003.08.004
KS Tung, YH Lou, AM Luo, J Ang, Contraceptive vaccine assessment based on a murine ZP3 mini-autoantigen Reproduction, Fertility and Development. ,vol. 6, pp. 349- 355 ,(1994) , 10.1071/RD9940349