A Canalicular Multispecific Organic Anion Transporter (cMOAT) Antisense cDNA Enhances Drug Sensitivity in Human Hepatic Cancer Cells

摘要: The human cMOAT gene encodes a membrane protein involved in the ATP-dependent transport of hydrophobic compounds. To determine whether is associated with drug sensitivity, we transfected an expression vector containing antisense cDNA into HepG2 hepatic cancer cell line. We observed reduction protein, as well enhanced level glutathione, transfectants. transfectants displayed increased sensitivity to cisplatin, vincristine, doxorubicin, and camptothecin derivatives, (4S)-4,11-diethyl-4-hydroxy-9-[(4-piperidinopiperidino)carbonyl oxy]dione hydrochloride triethydrate 7-ethyl-10-hydroxycamptothecin, but not etoposide, 3-[4-amino-2-methyl-5-pyrimidinyl)methyl]-1-(2-chloroethyl)-1-nitrosoure a, 5-fluorouracil, mitomycin C. Results suggest that levels are inversely correlated those its related genes may be cells certain anticancer agents.