IN VITRO SURVIVAL OF MURINE MORULAE AFTER QUICK FREEZING IN THE PRESENCE OF CHEMICALLY DEFINED MACROMOLECULES AND DIFFERENT CRYOPROTECTANTS

摘要: We studied the ability of frozen-thawed mouse morulae to develop in vitro when cryoprotectant proteins were substituted with one following nonorganic macromolecules: polyvinyl alcohol (PVA), pyrrolidone (PVP), and ficoll. also determined how these agents interacted 3 different cryoprotectants: glycerol (GLY), propylene glycol (PG), ethylene (EG). The influence both above factors was measured on basis post-thaw morphological appearance, percentage development expanded blastocyst stage total cell count. Morulae (n=950) collected from superovulated mice. Those classified as good or excellent distributed among 12 freezing solutions, obtained by combining cryoprotectants 4 macromolecules (the mentioned above, plus a control 5% fetal calf serum) phosphate buffered saline (PBS). Embryos frozen PVA, PVP ficoll tended be little difficult recover straws. Development significantly lower (P<0.05) (43.6%) than (79.5%) (76.1%). Polyvinyl provided higher survival rate combined (90.3) (95.0), but it glycol, only 56.5% embryos survived after thawing. A positive interaction observed between PVA results indicate that serum could successfully for any chemically defined macromolecules. However, our findings suggest use PG should avoided are using quick method.