The utility of CD44, CD117 and CD133 in identification of cancer stem cells (CSC) in oral squamous cell carcinomas (OSCC).
作者： D Pirici , Cl Mărgăritescu , Cristiana Simionescu , A Stepan
DOI:
关键词:
摘要: One of the theories regarding oral carcinogenesis is that tumor growth dependent on cancer stem cells (CSCs) have capacity self-renewal and giving rise to more differentiated cells, like do in normal tissues. The most used methods CSCs isolation are based their identification expression different cell surface markers. markers qualified for this purpose been described originally studies involving hematopoietic or embryonic cells. Thus, we were interested study formalin fixed paraffin embedded tissue samples from squamous carcinoma (OSCC). We investigated by immunohistochemistry thirty OSCCs with degrees differentiation locations. establish tissular localization pattern expressing CD44, CD133 CD117 tumoral samples. results indicated exception other two expressed only stromal When looked at origin (by double cytokeratins AE1-AE3, vimentin CD34) concluded they mesenchymal nature. Also, proved some these also co-expressed CD44 but negative CK5÷6. Moreover, positive especially had reactivity tryptase showing mast In conclusion, our has limited utility identifying CSCs, while appears be
参考文章(57)
Sheri Miraglia, Wayne Godfrey, Amy H. Yin, Kristin Atkins, Roger Warnke, Jeannine T. Holden, Robert A. Bray, Edmund K. Waller, David W. Buck, A novel five-transmembrane hematopoietic stem cell antigen: isolation, characterization, and molecular cloning. Blood. ,vol. 90, pp. 5013- 5021 ,(1997) , 10.1182/BLOOD.V90.12.5013
Y Li, P Heldin, Hyaluronan production increases the malignant properties of mesothelioma cells. British Journal of Cancer. ,vol. 85, pp. 600- 607 ,(2001) , 10.1054/BJOC.2001.1922
P. Kleihues, B. W. Stewart, World Cancer Report ,(2003)
Gang Shen, Fang Shen, Zhuojin Shi, Weiguo Liu, Weiwei Hu, Xuesheng Zheng, Liang Wen, Xiaofeng Yang, Identification of cancer stem-like cells in the C6 glioma cell line and the limitation of current identification methods In Vitro Cellular & Developmental Biology – Animal. ,vol. 44, pp. 280- 289 ,(2008) , 10.1007/S11626-008-9115-Z
D OLIVEIRA, Expression of CD44 variant exons by normal oral epithelia. Oral Oncology. ,vol. 33, pp. 260- 262 ,(1997) , 10.1016/S0964-1955(97)00081-X
Shih-Hwa Chiou, Cheng-Chia Yu, Chi-Yang Huang, Shu-Chun Lin, Chung-Ji Liu, Tung-Hu Tsai, Shiu-Huey Chou, Chian-Shiu Chien, Hung-Hai Ku, Jeng-Fan Lo, Positive correlations of Oct-4 and Nanog in oral cancer stem-like cells and high-grade oral squamous cell carcinoma. Clinical Cancer Research. ,vol. 14, pp. 4085- 4095 ,(2008) , 10.1158/1078-0432.CCR-07-4404
Archangel Levi Omara-Opyene, Jingxin Qiu, Girish V Shah, Kenneth A Iczkowski, Prostate cancer invasion is influenced more by expression of a CD44 isoform including variant 9 than by Muc18. Laboratory Investigation. ,vol. 84, pp. 894- 907 ,(2004) , 10.1038/LABINVEST.3700112
Qunzhou Zhang, Shihong Shi, Yun Yen, Jimmy Brown, Joseph Quoc Ta, Anh D. Le, A subpopulation of CD133+ cancer stem-like cells characterized in human oral squamous cell carcinoma confer resistance to chemotherapy Cancer Letters. ,vol. 289, pp. 151- 160 ,(2010) , 10.1016/J.CANLET.2009.08.010
M. E. Prince, R. Sivanandan, A. Kaczorowski, G. T. Wolf, M. J. Kaplan, P. Dalerba, I. L. Weissman, M. F. Clarke, L. E. Ailles, Identification of a subpopulation of cells with cancer stem cell properties in head and neck squamous cell carcinoma Proceedings of the National Academy of Sciences of the United States of America. ,vol. 104, pp. 973- 978 ,(2007) , 10.1073/PNAS.0610117104
A Eramo, F Lotti, G Sette, E Pilozzi, M Biffoni, A Di Virgilio, C Conticello, L Ruco, C Peschle, R De Maria, Identification and expansion of the tumorigenic lung cancer stem cell population. Cell Death & Differentiation. ,vol. 15, pp. 504- 514 ,(2008) , 10.1038/SJ.CDD.4402283