Identification of cancer stem-like cells in the C6 glioma cell line and the limitation of current identification methods

摘要: The cancer stem cell (CSC) hypothesis has provided insights into the initiation and recurrence of brain tumor. Specific identification targeted elimination these CSCs within tumor mass represents a promising therapeutic strategy for refractory tumors. In this study, we attempted to identify in rat C6 glioma line by three different methods. It is interesting note that single-cell clonal analysis showed most cells are stem-like with characteristics self-renewal, multilineage differentiation potentials vitro, tumorigenic capacity vivo. surprising CD133 failed total population line, since both (+) (−) have fractions. Moreover, Hoechst 33342 staining, which used flow cytometry isolate side (SP), was found be harmful cells. Therefore, non-SP may also harbor These results imply limitation using current methods underscore importance defining genetic molecular basis CSCs.