CD133(+) and CD133(-) glioblastoma-derived cancer stem cells show differential growth characteristics and molecular profiles.

摘要: Although glioblastomas show the same histologic phenotype, biological hallmarks such as growth and differentiation properties vary considerably between individual cases. To investigate whether different subtypes of might originate from cells origin, we cultured tumor 22 under medium conditions favoring neural cancer stem (CSC). Secondary glioblastoma (n = 7)-derived did not any in used, suggesting absence cell-like cells. In contrast, 11/15 primary contained a significant CD133(+) subpopulation that displayed neurosphere-like, nonadherent asymmetrical cell divisions yielding expressing markers characteristic for all three lineages. Four 15 lines derived grew adherently vitro were driven by CD133(-) fulfilled criteria. Both similarly tumorigenic nude mice vivo. Clinically, characterized lower proliferation index, whereas glial fibrillary acidic protein staining was similar. GeneArray analysis revealed 117 genes to be differentially expressed these two subtypes. Together, our data provide first evidence CSC maintain only subset glioblastomas. The remainder stems previously unknown with apparent but distinct molecular profiles characteristics