Dynamism and regulation of the stator, the energy conversion complex of the bacterial flagellar motor.

作者: Seiji Kojima

DOI: 10.1016/J.MIB.2015.07.015

关键词:

摘要: Many motile bacteria swim by rotating their motility organ, the flagellum. Rotation of flagellum is driven a motor at its base, and torque generated rotor–stator interaction coupled with specific ion flow through channel in stator. Because stator works as an energy-conversion complex motor, understanding functional mechanism critically important. But characterization has been hampered due to difficulty isolating from membrane. Recently, successful new approaches for studying have reported that reveal novel properties. Two those, visualization vivo behavior units using fluorescently tagged proteins structure-guided analyses soluble region stator, are summarized this short review.

参考文章(49)
Na Li, Seiji Kojima, Michio Homma, Sodium-driven motor of the polar flagellum in marine bacteria Vibrio Genes to Cells. ,vol. 16, pp. 985- 999 ,(2011) , 10.1111/J.1365-2443.2011.01545.X
Akihiro Kawamoto, Yusuke V. Morimoto, Tomoko Miyata, Tohru Minamino, Kelly T. Hughes, Takayuki Kato, Keiichi Namba, Common and distinct structural features of Salmonella injectisome and flagellar basal body Scientific Reports. ,vol. 3, pp. 3369- 3369 ,(2013) , 10.1038/SREP03369
Seiji Kojima, David F. Blair, Solubilization and purification of the MotA/MotB complex of Escherichia coli. Biochemistry. ,vol. 43, pp. 26- 34 ,(2004) , 10.1021/BI035405L
Edan R. Hosking, Christian Vogt, Evert P. Bakker, Michael D. Manson, The Escherichia coli MotAB Proton Channel Unplugged Journal of Molecular Biology. ,vol. 364, pp. 921- 937 ,(2006) , 10.1016/J.JMB.2006.09.035
S. Kojima, A. Shinohara, H. Terashima, T. Yakushi, M. Sakuma, M. Homma, K. Namba, K. Imada, Insights into the stator assembly of the Vibrio flagellar motor from the crystal structure of MotY Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 7696- 7701 ,(2008) , 10.1073/PNAS.0800308105
Anja Paulick, Andrea Koerdt, Jürgen Lassak, Stuart Huntley, Ina Wilms, Franz Narberhaus, Kai M. Thormann, Two different stator systems drive a single polar flagellum in Shewanella oneidensis MR-1. Molecular Microbiology. ,vol. 71, pp. 836- 850 ,(2009) , 10.1111/J.1365-2958.2008.06570.X
Shiwei Zhu, Masato Takao, Na Li, Mayuko Sakuma, Yuuki Nishino, Michio Homma, Seiji Kojima, Katsumi Imada, Conformational change in the periplamic region of the flagellar stator coupled with the assembly around the rotor. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 111, pp. 13523- 13528 ,(2014) , 10.1073/PNAS.1324201111