Dynamic Events Are Differently Mediated by Microfilaments, Microtubules, and Mitogen-Activated Protein Kinase During Porcine Oocyte Maturation and Fertilization In Vitro

摘要: The role of microfilaments, microtubules, and mitogen-activated protein (MAP) kinase in regulation several important dynamic events porcine oocyte maturation fertilization is described. Fluorescently labeled cortical granules were visualized using either epifluorescence microscopy or laser scanning confocal microscopy. Mitogen-activated phosphorylation was revealed by Western immunoblotting. We showed that 1) microfilament disruption did not affect meiosis resumption metaphase I meiotic apparatus formation but inhibited further cell cycle progression (chromosome separation) even though MAP phosphorylated; 2) granule (CG) migration driven microfilaments (but microtubules), once the chromosomes CGs localized beneath oolemma their anchorage to cortex independent microtubules; 3) neither nor microtubules involved CG exocytosis during activation; 4) sperm incorporation mediated while pronuclear (PN) syngamy controlled rather than microfilaments; 5) spindle microtubule organization temporally correlated with phosphorylation, extensive aster required for PN apposition occurred absence 6) change when disrupted nocodazole cytoplasmic asters induced taxol. present study suggests cytoskeleton similar rodents, mechanisms pig resemble those lower vertebrates.